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Model 1420 Video Microscope


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  • Inverted tabletop Microscope
  • x=y Transverse Resolution 10 µm
  • Color Cam 640 x 480 pixel, 490 lines res
  • B&W Cam 640 x 480 pixel, 600 lines res
  • Objective 10x semi-plan
  • Illuminator Module 4 channel array
The Model 1420 Scanning Video Microscope is a research-grade, inverted fluorescence video microscope for applications in many fields. This general purpose microscope combines some of the most advanced features with portability and value pricing. With a synchronously pulsed illuminator, sensitive 30-fps camera, and powerful video analysis software, the Model 1420 workhorse for many academic, industrial, R&D and research activities. Extensive filter, camera, illumination and objectives options let you tailor the system for your research. Motorized traverse and focus, and software-configurable image-based "soft triggers" make the Model 1420 perfect for µTAS prototypes and miniature process automation. The Model 1420 is sized and priced to be dedicated to a single user or experiment, even in a crowded BSL-2 lab. The Model 1420 microscope excels precisely where it counts: getting high-quality experimental data onto a hard drive.

Application Ideas

  • Embed the Model 1420 and automate your µTAS.
  • Motorized focus and x-y traverse can be fully automated using LabVIEW and simple software developers kit (C++)
  • Standard composite and S-Video analog outputs and USB and RS232 digital ports simplify electronic interfacing
  • Motionless sample stage simplifies physical interfacing
  • Losslessly record data to disk
  • Post-save and instantly replay what just happened so you never “just missed it”
  • Probe velocity by real-time particle image velocimetry
  • Probe regions with real-time image processing
  • Trigger devices and recording by hardware, software, and real-time image probes
  • Sensitive 30 fps color or black & white video cameras support low-light imaging
  • Synchronous pulsed illuminator fights blur and photo-bleaching
  • Bottom up viewing and illumination and a motionless sample stage allow unhindered and unperturbed microsystem access
  • Simple coordination with external apparatus µTAS research and prototyping
  • Gene and protein chip reading
  • General miniature lab automation
  • Closed-loop microsystem control
  • Automated methods development
  • Array assays
  • Forensics
  • Blood analysis
  • Particle image velocimetry (PIV)

Software Features

Losslessly record data to disk (640 x 480 pixels) - Post-save and instant replay so you never miss an event - Auto-acquisition by hardware, software, or real-time image processing - Track and trigger by local intensity or color - Analyze and trigger by high-resolution PIV.

Upgradeable

As with many of Berkeley Nucleonics’ products, the field upgrades and future features will integrate seamlessly into your Model 1420. We can offer simple options like switching between color and black & white cameras, or trying different illuminator colors. For more advanced customization, see our snap-in optical modules which are customizable or preconfigured for one or more fluorophores. The stage cover is machinable and is easily swapped to support custom fixturing. One-click firmware and software upgrades provide the latest features and capabilities.

The Model 1420 is compact (just 21 cm wide x 27 cm long x 9 cm tall) but scans up to 75 mm x 50 mm. With its size and price point, the Model 1420 is ideal for dedicating to a single user or experiment.

Camera Modules

The snap-in camera modules for the Model 1420 include a CCD camera, optional fluorescence filter and threading for standard DIN microscope objective:
Black and White Module: High sensitivity analog black & white camera with 640 x 480 pixels, 600 lines of resolution, 30 frames per second.

Color Module: Bayer-pattern analog color camera with 640 x 480 pixels, 490 lines of resolution, 30 frames per second, S-Video output.

Illumination Options

The Model 1420 illuminator offers four selectable LED channels which can be used independently or simultaneously. You can specify the color for each channel from the following LED wavelengths:
Blue: 464-476 nm
Green: 520-535 nm
Red: 615-636 nm
White
Custom wavelengths are also available.

Filter Options

The SVM uses 12.5 mm diameter long pass filters. Standard filters from 515 nm to 610 nm are available.

Power Numerical Aperture Working Distance Focal Length Field of View
4X 0.10 15.97 mm 31.0 mm 1.5 x 1.5 mm
10X 0.25 6.3 mm 16.76 mm 0.6 x 0.6 mm
20X 0.40 3.3 mm 8.55 mm 0.3 x 0.3 mm

Stage Plate Options

The standard stainless steel stage plate has a 50 x 75 mm opening for easy sample viewing. An optional plate is available with two openings of 20 x 32 mm and 22 x 66 mm for smaller chips.

Suggested Options for Typical Applications

The Model 1420 illuminator and filter options can be configured for particular applications. For example, when working with fluorescein particles the Model 1420 illuminator can be equipped with one channel of white LEDs (to view the channels and chip during setup) and three blue LED channels (to excite the fluorescein particles). A 530 nm filter and black & white camera are also suggested.

Application Illuminator Camera Module Filter
Fluorescein 3 blue channels & 1 white channel Black & White 530 nm
Rhodamine 3 green channels & 1 white channel Black & White 515 or 530 nm
Colored Dyes 1 blue, 1 green, 1 red, & 1 white channel Color None

Model 1420 FAQ

Can I incorporate active microsystems for viewing with the SVM?

Yes. Contact us about bonded-port connectors, which let you join microsystems to chips or slides for viewing in the SVM. We can offer you a document entitled “How to attach a bonded port connector to a glass slide.

How do I image my micro-channels in color?

1. Ensure that you have a color camera module installed (check the label on the front of the camera module). The Model 1420 comes standard with a B&W camera module. Additional camera modules can be purchased.

2. Verify that the color format is set to RGB by choosing Video>Color format>24 bit RGB.

3. Choose View>Video Options>Analog settings... and set the saturation to 100% of scale.

Does the SVM include any software for image processing?

The uScope software, which comes with the SVM, includes some real-time image processing, such as Intensity Over a Region, and PIV. These measurements can be saved to disk and analyzed later.

Can I use PIV probes with the SVM?

Yes. The included uScope software lets you place multiple probes in the flow and control their individual settings. Contact us for a user note explaining their use.

Which video card should I use with my Model 1420?

For a desktop computer: we recommend the Hauppauge Impact VCB video input adapter - PCI (558).

For a laptop: USB video card we recommend is the ADS TECH INSTANT VIDEOMPX (USBAV-708).

One card is included with your MODEL 1420 (your choice, at the time of purchase). Additional units can be purchased from BNC or from many electronics retailers.

What functionality do I gain with the Software Developers' Kit?

The SDK allows you to move the stage and change illuminator settings automatically.

uScope Software V1.013

uScope software is a complete solution for hands-off control of the Model 1420, video recording and real-time video image processing and metrology. The full functionality of this software, including image processing, is currently unlocked only when a Model 1420 is detected. If you do not own a Model 1420 you may contact BNC to request a software license or trial with all capabilities unlocked.

LabView Drivers

This driver allows you to control the Model 1420 functions through National Instruments' LabView software. Download the file, then copy it into your LabView VI library directory.

Software Developer's Kit

To obtain the Model 1420 software developer's kit (C, C++), or if you have further questions, give us a call, we are happy to discuss your applications. 800-234-7858

Model 1420 Specifications

Illuminator Module
Four-channel array of 24 high-brightness LEDs.

Available modules:

  • LED-B — 3 blue channels and one white channel
  • LED-G — 3 green channels and one white channel
  • LED-R — 3 red channels and one white channel
  • LED-W — 4 white channels
  • LED-X — one channel each of blue, green, red and white LEDs.
  • Custom modules also available.

Trigger output available for driving external LED or laser illuminator.
Property Range Resolution:

  • x-traverse 50 mm 10 µm
  • y-traverse 75 mm 10 µm
  • focus 8 mm 1 µm

INPUTS & OUTPUTS
Analog (camera dependent):

  • Composite video (NTSC)
  • S Video

Digital (used for synchronization and coordination):

  • 4 programmable inputs
  • 3 programmable outputs
  • External LED/Laser illuminator trigger/driver
  • RS232 or USB control interface

uScope™ software included:

  • Illuminator control
  • x-ytraverse, and focus control
  • Save and process videos and images
  • Particle image velocimetry

LabVIEW drivers available Software developer’s kit (C, C++) included
Requirements:

  • PC-compatible computer
  • Windows® 2000, XP or later
  • PCI slot or USB2.0 port

x-y TRAVERSE

Motorized x-y traverse and focus are controlled through the software and the front panel.
Dimensions:

  • Width: 210 mm (8.3”)
  • Length: 270 mm (10.5”)
  • Height: 85 mm (3.3”)

Material:

  • Enclosure is constructed of black Teflon® coated steel

Weight:

  • 2.8 kg

Power requirements:

  • Voltage 90–264 VAC, 47–63 Hz
  • Current 0.5 A

Mounting:

  • Four 8-32 threaded mounting holes on 177.8 mm (7.0”) x 228.6 mm (9.0”) rectangle

SAMPLE STAGE

A fixed sample stage is attached to the top surface of the enclosure. It is constructed of black Delrin with a stainless steel top plate. The sample stage can be removed and machined for custom applications.

Dimensions:

  • Width: 140 mm (5.5”)
  • Length: 175 mm (7.0”)
  • Height: 12 mm (0.5”)
  • Opening: 55 mm x 80 mm

CAMERA MODULE
Snap-in module with CCD camera, optional fluorescence filter and threading for standard DIN microscope objective.

Available modules:
RS-170-BW: (High sensitivity analog black & white camera with 640 x 480 pixels,600 lines of resolution, 30 frames per second)
RS-170-C: (Bayer-pattern analog color camera with 640 x 480 pixels,490 lines of resolution, 30 frames per second, S-Video output)
10X semi-plan (standard)
4X and 20X (optional)

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